Figure 5.
The microbiome between healthy and leukemic Pax5+/−mice is distinct. (A) After antibiotic treatment, the beta diversity of the microbiomes shifted in a genotype-dependent manner and reconstituted over time toward a similar end point (Pax5+/–, yellow; WT, blue). Pairwise unweighted UniFrac distances (beta diversity) were computed for all samples. Distance metric was ordinated into 3D via PCoA and visualized using EMPeror. Axes indicate percentages of explained variance. Cones and spheres were used to visualize microbiomes of Pax5+/– and WT mice, respectively. Icon size indicates disease status, with larger icons for pB-ALL mice and smaller icons for healthy mice. Samples of the initial time point (darkest red), at transfer from SPF facilities to CFs before antibiotics treatment, clustered in the light blue pre-abx region. Samples with sufficient bacterial reads from the group that had 8 weeks of antibiotic treatment clustered in the green abx region. Samples shifted back after antibiotic treatment in a genotype-dependent manner (Pax5+/–, yellow; WT, blue) and reconstituted over time toward a similar end point. Their end point was significantly shifted from pre-abx, which was not the case for samples of mice that were housed in SPF facilities (orange trajectory). (B-C) Microbial V4-16S signatures (V4-ASVs) differentiated between healthy (B) and preleukemic (C) Pax5+/– mice. Shown are mean V4-ASV compositions for the top 13 differentially abundant (discrete FDR) features of all samples stratified by disease status. Samples were grouped by time point (x-axis) and visualized as 1 stacked bar each. A naive Bayesian classifier was applied against the Greengenes 13.8 reference to assign taxonomic labels to the V4-ASVs, which are annotated in the legend.

The microbiome between healthy and leukemic Pax5+/−mice is distinct. (A) After antibiotic treatment, the beta diversity of the microbiomes shifted in a genotype-dependent manner and reconstituted over time toward a similar end point (Pax5+/–, yellow; WT, blue). Pairwise unweighted UniFrac distances (beta diversity) were computed for all samples. Distance metric was ordinated into 3D via PCoA and visualized using EMPeror. Axes indicate percentages of explained variance. Cones and spheres were used to visualize microbiomes of Pax5+/– and WT mice, respectively. Icon size indicates disease status, with larger icons for pB-ALL mice and smaller icons for healthy mice. Samples of the initial time point (darkest red), at transfer from SPF facilities to CFs before antibiotics treatment, clustered in the light blue pre-abx region. Samples with sufficient bacterial reads from the group that had 8 weeks of antibiotic treatment clustered in the green abx region. Samples shifted back after antibiotic treatment in a genotype-dependent manner (Pax5+/–, yellow; WT, blue) and reconstituted over time toward a similar end point. Their end point was significantly shifted from pre-abx, which was not the case for samples of mice that were housed in SPF facilities (orange trajectory). (B-C) Microbial V4-16S signatures (V4-ASVs) differentiated between healthy (B) and preleukemic (C) Pax5+/– mice. Shown are mean V4-ASV compositions for the top 13 differentially abundant (discrete FDR) features of all samples stratified by disease status. Samples were grouped by time point (x-axis) and visualized as 1 stacked bar each. A naive Bayesian classifier was applied against the Greengenes 13.8 reference to assign taxonomic labels to the V4-ASVs, which are annotated in the legend.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal