Prevalence of MRD by leukemia burden and 5-year DFS subdivided by EOI1 leukemia burden. MRD prevalence was subdivided by proportion of MRD after EOI1 (A) (0.02%∼0.1% [25 patients], 0.1%∼1% [52 patients], 1%∼5% [79 patients], and ≥5% [107 patients]) among 263 MRD⁺ patients enrolled in AAML0531. (B) The correlation of 5-year DFS and the amount of MRD at EOI1 is shown. A total of 521 patients enrolled in AAML0531 were MRD⁻ at EOI1. The time to median DFS was considered for each category: ≥5% was 40 days, 1% to 5% was 222.5 days, 0.1% to 1% was 185 days, and MRD 0.02% to 0.1% was 246 days.