Figure 2.
Characterization of the secreted reporter with the missense mutations in the signal peptide and propeptide. SCSR protein (A) and STR protein (B) were normalized to Metridia luciferase and expressed relative to wild-type FIX-PC. The error bars indicate the standard deviation from 3 biological replicates. The dashed blue and red lines show 40% and 5% level of wild-type FIX-PC reporter, respectively. (C) Normalized RC/T. The ratio was renormalized by RC/T of wild-type FIX-PC, which is defined as “1”. All error propagations were calculated accordingly. The black, green, and violet dashed lines show 1.2-fold, 0.8-fold, and 0.2-fold wild-type RC/T, respectively.

Characterization of the secreted reporter with the missense mutations in the signal peptide and propeptide. SCSR protein (A) and STR protein (B) were normalized to Metridia luciferase and expressed relative to wild-type FIX-PC. The error bars indicate the standard deviation from 3 biological replicates. The dashed blue and red lines show 40% and 5% level of wild-type FIX-PC reporter, respectively. (C) Normalized RC/T. The ratio was renormalized by RC/T of wild-type FIX-PC, which is defined as “1”. All error propagations were calculated accordingly. The black, green, and violet dashed lines show 1.2-fold, 0.8-fold, and 0.2-fold wild-type RC/T, respectively.

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