Altered posttranslational modifications of microtubules in spread DKO MKs. (A) MKs were allowed to spread on Horm collagen and stained for acetylated or detyrosinated microtubules. (B) Fluorescence intensity of acetylated or detyrosinated microtubules was assessed using ImageJ Software. Values were normalized to α-tubulin. Values are mean ± SD (n = 3). Unpaired, 2-tailed Student t test. **P < .01; ***P < .001. (C) MKs were incubated with 500 µm colchicine for 30 minutes at 37°C, washed, resuspended in TPO-conditioned medium, and allowed to spread on Horm collagen. Spreading area was analyzed using ImageJ software. At least 30 MKs were analyzed per animal. Values are mean ± SD (n = 3). One-way ANOVA with Sidak correction for multiple comparisons. *P < .05; ***P < .001. colch, colchicine.