Growth kinetics and cell cycle analysis of M1, M1myc, M1Egr-1, and M1Egr-1/myc (clones 6 and 7) cells treated with IL-6. (A) Cells were seeded at 0.05 × 106 cells/mL with IL-6 (50 ng/mL). At indicated time points viable cell numbers were determined using a hemocytometer (see “Materials and methods”). Results are the average of 3 independent experiments with an SD up to 15%. Applying the Student t test to the day 4 data, the differences between M1myc and either M1Egr-1/myc6 or M1Egr-1/myc7 were P < .05 (significant), between M1Egr-1 and either M1Egr-1/myc6 or M1Egr-1/myc7 P < .05 (significant), and between M1 and M1Egr-1 P > .05 (not significant). (B) Cells were seeded at 0.05 × 106 cells/mL with or without IL-6 (50 ng/mL). After 3 days cell cycle analysis was performed using FACS analysis, as described in “Materials and methods.” This is a representative experiment, which was repeated a total of 4 times. (C) Percent cells adhering to the surface of the tissue culture dish was determined 3 days following seeding 0.05 × 106 cells/mL in the presence of IL-6 (50 ng/mL). Results are the average of 3 experiments, with an SD up to 15%. The differences between M1myc and M1Egr-1/myc were P > .1 (not significant), between M1 and M1myc P < .05 (significant), between M1 and M1Egr-1/myc P < .05 (significant), and between M1 and M1Egr-1 P > .05 (not significant).
