![Figure 4. Effect of in vitro neutralization of CXCL12 and CXCR4 on hu/SCID lymphoma cell proliferation and survival. (A) Ex vivo–obtained hu/SCID lymphoma cells were cultured for 48 hours with or without TC14012 (50 to 100 μM), neutralizing anti-CXCL12 Abs (1:20), or appropriate controls. Each result is representative of the mean counts per minute (cpm) ± SD of triplicate wells after a 48-hour pulse with [methyl-3H]thymidine. This analysis is representative of 3 separate experiments with different tumors with similar results; the single asterisk denotes a significant difference compared with untreated cells (P < .05). (B) Ex vivo–obtained hu/SCID lymphoma cells were cultured for 48 hours with or without TC14012 (50 to 100 μM), neutralizing anti-CXCL12 Abs (1:20), or appropriate controls. Cells were then harvested and labeled with PI and FITC-conjugated annexin V before being analyzed by flow cytometry. Data are shown as the mean percent change relative to control. One representative experiment of 3 performed is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/3/10.1182_blood-2004-03-0799/6/zh80030573180004.jpeg?Expires=1756268004&Signature=2yWO~9qrwGUG54zXalEQagYp08u7c9iGr0CXP258bCPCGwZEjwraJUffnT1in5VI9PpJ~2WUSP6ljdJgMe1t9nsYaOdT37V-01SpR~75yNKC-w4NQh7ya9ZA59aQYeaKpc0sX0TAO0Ul7K7dkzmfOgDmy5ixVbVPThUKMUXBIU~tyulhv~DZSfEwbdCAzhUv9JoiiV36DSBEEuP311rbMRilYwQF1qaGEAtN1xweKhsMcNkMziOkBopAGA~X26HlTYYDFlKs8uvfWCb9eXI2kU5YAc0yKg628gOgp5v0RGbyWhIbvTtk1WVoFIUES1TUIujiGN6JZw-rG6aelbS3uQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of in vitro neutralization of CXCL12 and CXCR4 on hu/SCID lymphoma cell proliferation and survival. (A) Ex vivo–obtained hu/SCID lymphoma cells were cultured for 48 hours with or without TC14012 (50 to 100 μM), neutralizing anti-CXCL12 Abs (1:20), or appropriate controls. Each result is representative of the mean counts per minute (cpm) ± SD of triplicate wells after a 48-hour pulse with [methyl-3H]thymidine. This analysis is representative of 3 separate experiments with different tumors with similar results; the single asterisk denotes a significant difference compared with untreated cells (P < .05). (B) Ex vivo–obtained hu/SCID lymphoma cells were cultured for 48 hours with or without TC14012 (50 to 100 μM), neutralizing anti-CXCL12 Abs (1:20), or appropriate controls. Cells were then harvested and labeled with PI and FITC-conjugated annexin V before being analyzed by flow cytometry. Data are shown as the mean percent change relative to control. One representative experiment of 3 performed is shown.
