Ad5F35-TK delivered by activated E1-CTLs killed LCLs. E1-CTLs (C-RE) were transduced (1000 vp per cell) with an Ad5F35-TK vector and cultured with autologous and allogeneic LCLs. At 24 hours after coculture, the CTLs (C-RE-AT) were removed by fluorescence-activated cell sorting and the LCLs counted. The same numbers of LCL cells were cultured with or without ganciclovir for 3 days. For each condition, 6 identical wells were used. The viability of the cells was then measured with an adenosine triphosphate (ATP) assay. Control cells were LCLs directly infected with Ad5F35-TK vector. Results are reported as the percentage of dead cells. Results are shown as mean ± SD.