Secondary granule LTF release is equivalent in WT and Rac2^-/- neutrophils. BMNs were stimulated with increasing doses of (A) fMLP (after CB preincubation). Release was significant (P < .05) at the highest dose of agonist. (B) Comparison of the effects of CB, CB with 40 μM fMLP, PMA (500 ng/mL), fibronectin adhesion (1 hour), or fibronectin (1 hour) followed by 5 μM fMLP on LTF release determined by immunoassay. Error bars indicate SEM.