Figure 1.
Figure 1. Sequence and GeneScan analysis of DNA from purified lymphocyte fractions. (Ai) WASP exon 10 genomic sequence surrounding the 1305insG mutation. DNA was isolated from sorted, WASP-negative T cells3 of patient II-1. The original 1305insG mutation is highlighted by a red box, and the DraII recognition sequence is underlined. (Aii) Sequence analysis of the same genomic region as in (i) performed on DNA isolated from sorted, WASP-positive T cells and showing the presence of Δ19bp. (Aiii) Sequence of the gel-purified Δ19bp fragment amplified from DNA obtained from sorted B cells after DraII digestion. (B) GeneScan analysis of specific 1305insG (insG) and Δ19bp (Δ19) fragments obtained from DNA isolated from purified CD3+ T cells and CD20+ B cells before and after digestion with DraII.

Sequence and GeneScan analysis of DNA from purified lymphocyte fractions. (Ai) WASP exon 10 genomic sequence surrounding the 1305insG mutation. DNA was isolated from sorted, WASP-negative T cells3  of patient II-1. The original 1305insG mutation is highlighted by a red box, and the DraII recognition sequence is underlined. (Aii) Sequence analysis of the same genomic region as in (i) performed on DNA isolated from sorted, WASP-positive T cells and showing the presence of Δ19bp. (Aiii) Sequence of the gel-purified Δ19bp fragment amplified from DNA obtained from sorted B cells after DraII digestion. (B) GeneScan analysis of specific 1305insG (insG) and Δ19bp (Δ19) fragments obtained from DNA isolated from purified CD3+ T cells and CD20+ B cells before and after digestion with DraII.

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