Expression of differentiation markers in full-length or ΔNT mGATA-1 virus–infected MGS cells. Differentiation of MGS cells with retroviral expression of GATA-1. (A) MGS cells were infected with retrovirus (MSCV) containing full-length mGATA-1 (MSCV–mGATA-1), or MSCV alone, and cultured without the addition of cytokines. The expression of CD34, glycophorin A, CD41a, CD61, CD13, CD3, and CD19 markers on the cell surface was examined by FACS. Upper panels show MGS cells without MSCV infection, while middle panels show MGS cells infected with empty MSCV vector. Lower panels show MGS cells infected with MSCV–mGATA-1 expressing full-length mGATA-1. (B) MGS cells infected with MSCV–mGATA-1 (GATA-1), ΔNT (GATA-1 dNT), or MSCV vector were cultured without cytokines and the expression of CD34, glycophorin A, CD41a, and CD61 was analyzed. The results of 2 separate experiments (no. 1 and no. 2) are shown. Note that the increase of glycophorin A⁺/CD34–cells is observed only in the MSCV–mGATA-1–infected MGS cells.