Fig. 2.
Fig. 2. Transient expression of wild-type and mutant FV protein in COS-1 cells. / pMT2/FV (wild type; ■), pMT2/FV-Arg2074Cys (mutant; ▪), or equimolar amounts of both plasmids (heterozygous condition; ░) were transiently transfected in COS-1 cells. Equal numbers of cells and equal amounts of plasmids were used in transfection experiments, as described in “Materials and methods.” (A) Antigen levels of recombinant FV were measured both in conditioned media and in the corresponding cell lysates by an EIA assay. (B) The specific activities of recombinant FV were determined by calculating the ratio between FV activity (measured by a functional assay based on the prothrombin time) and FV antigen levels, both assayed in about 50 × concentrated media. Bars represent means ± standard deviations of 3 independent experiments, each performed in duplicate. The mean value of wild-type FV was set as 100%.

Transient expression of wild-type and mutant FV protein in COS-1 cells.

pMT2/FV (wild type; ■), pMT2/FV-Arg2074Cys (mutant; ▪), or equimolar amounts of both plasmids (heterozygous condition; ░) were transiently transfected in COS-1 cells. Equal numbers of cells and equal amounts of plasmids were used in transfection experiments, as described in “Materials and methods.” (A) Antigen levels of recombinant FV were measured both in conditioned media and in the corresponding cell lysates by an EIA assay. (B) The specific activities of recombinant FV were determined by calculating the ratio between FV activity (measured by a functional assay based on the prothrombin time) and FV antigen levels, both assayed in about 50 × concentrated media. Bars represent means ± standard deviations of 3 independent experiments, each performed in duplicate. The mean value of wild-type FV was set as 100%.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal