Fig. 4.
Fig. 4. Epo (R103A) inhibits 125I-Epo binding. / 125I-Epo (at a final concentration of 0.132 nM) was incubated with 32D/EpoR WT cells in the presence of increasing amounts of unlabeled Epo (R103A). Total cell-associated 125I-Epo was measured. Binding in the presence of 3000-fold excess unlabeled Epo (to determine nonspecific binding) is shown in the solid circle. Determinations were done in quadruplicate with SD shown.

Epo (R103A) inhibits 125I-Epo binding.

125I-Epo (at a final concentration of 0.132 nM) was incubated with 32D/EpoR WT cells in the presence of increasing amounts of unlabeled Epo (R103A). Total cell-associated 125I-Epo was measured. Binding in the presence of 3000-fold excess unlabeled Epo (to determine nonspecific binding) is shown in the solid circle. Determinations were done in quadruplicate with SD shown.

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