Inhibitory effects of synthetic peptides on FVIII bindings to anhydro-APC and VWF.

Serial dilutions of synthetic peptides were mixed with FVIII prior to adding to immobilized anhydro-APC (A) or VWF (B). Bound FVIII was detected using peroxidase-conjugated MoAb JR8. The symbols used are as follows: P-1, ○; P-2, ●; P-3, ■; P-4, ▪; P-5, ▵; P-6, ▴. (C) Percent inhibition of FVIII binding to anhydro-APC or VWF by synthetic peptide (P-1–P-6) at the concentration of 200 μM