Fig. 4.
Stat5-RARα fusion proteins induce ATRA-insensitive interaction with SMRT.
(A) Cellular extracts from 293T cells expressing Gal4 fusions of Stat5(1-636)–RARα and RARα were incubated with radiolabeled SMRT and precipitated with a 2 × UAS oligo. SMRT could be detected in precipitates with Stat5(1-636)–RARα in the absence or presence of ATRA (10−5 M). In contrast, no SMRT binding was seen in the presence of ATRA with extracts containing RARα. (B) Western blot performed with 30% of precipitated proteins using an anti-Gal4 antibody showed equal binding of both Gal4-fusion proteins to the oligo.
