Fig. 7.
Expression of lysosomal protease cathepsin B is positively regulated by ICSBP.
(A) Cytosolic RNA was isolated from BMMs and analyzed by RT-PCR using specific primers for cathepsin B cDNA. (B) BMMs were cultured with 20 ng/mL CSF-1 and stimulated with 200 U/mL IFN-γ and 100 ng/mL LPS or mock-stimulated for 24 hours. Total cell extracts of 5 × 105 cells per lane were subjected to western blotting with an anti–cathepsin B antibody. Equal protein loading was confirmed by blotting with an anti-ICSBP antiserum detecting ICSBP and p80.
