Histologic examinations.
Histology of formalin-fixed liver sections from hemophilia B dogs (with FIX null mutation) Brad (A, D, G), Semillon (B, E, H), and Beech (C, F, I) treated with the AAV-(ApoE)4/hAAT-cFIX vector. The tissues were stained with hematoxylin and eosin (H&E, A-F) or Prussian blue (PB), a stain for detection of iron (G-I). Tissues from Brad and Semillon were taken by biopsy at week 27 and 32, respectively, and tissue from Beech was taken by necropsy at week 11. Beech had PK deficiency, a disease characterized by iron deposition in the liver as evident by the blue stain in panel I. Fibrosis (note fibrotic changes in panels C and F) is present in panels C and F but not in panels A, B, D, and E. Original magnification × 100 (A-C and G-I) or × 400 (D-F).
