Fig. 8.
Fig. 8. CD40 activation enhances VEGF promoter activity. / MM cells were first incubated overnight in 2% fetal bovine serum containing RPMI 1640 medium at 37°C. A 2.3-kb full-length human VEGF promoter-luciferase reporter (VEGF-Luc) was transduced into MM cells by adenoviruses. MM cells were cultured for 6 hours in the presence or absence of sCD40L, in duplicate wells for each condition. Luciferase activity was analyzed using equal amounts of total protein for each sample. Mock-transduced MM cells with Luc reporter without any VEGF promoter sequences (Luc) served as a negative control. Luciferase activity was consistently induced (4-fold for RPMI 8226, 5.5-fold for SV, and 6-fold for patient MM5 cells) in sCD40L-activated cells compared with unstimulated cells. Results are representative of 2 independent experiments performed in duplicate cultures. (■) RPMI 8226; (▪) SV; and () patient MM5 cells.

CD40 activation enhances VEGF promoter activity.

MM cells were first incubated overnight in 2% fetal bovine serum containing RPMI 1640 medium at 37°C. A 2.3-kb full-length human VEGF promoter-luciferase reporter (VEGF-Luc) was transduced into MM cells by adenoviruses. MM cells were cultured for 6 hours in the presence or absence of sCD40L, in duplicate wells for each condition. Luciferase activity was analyzed using equal amounts of total protein for each sample. Mock-transduced MM cells with Luc reporter without any VEGF promoter sequences (Luc) served as a negative control. Luciferase activity was consistently induced (4-fold for RPMI 8226, 5.5-fold for SV, and 6-fold for patient MM5 cells) in sCD40L-activated cells compared with unstimulated cells. Results are representative of 2 independent experiments performed in duplicate cultures. (■) RPMI 8226; (▪) SV; and () patient MM5 cells.

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