Fig. 6.
![Fig. 6. Neutralizing anti-VEGF mAb blocks CD40-induced MM cell migration. / MM cells were subjected to transmigration assay and exposed for 8 hours (RPMI 8226 cells) or 16 hours (SV cells) to CD40 activation by sCD40L (0 or 10 μg/mL [μM]), in the presence or absence of neutralizing anti-VEGF mAb (0-5 μg/mL [μM]) added to the lower chamber of the transwell. Migrated cells collected from the lower chamber were counted. Enhanced transmigration of cells induced by sCD40L was abrogated by neutralizing anti-VEGF mAb. (■) RPMI 8226; (▪) SV.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/4/10.1182_blood.v99.4.1419/6/h80422116006.jpeg?Expires=1750745980&Signature=O~0V3wI61tQOpyFX9Zp48-S0TDtqyjj2MbMBjj~W9szcobxOq3UR-EAmFmxe5Xud9gPtm6q6txSnaMp6GRuUS9ghrAu-ZQXAS484OCGyYbRKxHCOoncSLpU3GZUA4g2t3iwxEa6iYfOn7-e2uEMyv9AajR6DaK1cgq1nMInvofQOa4WrvRUr1jgJBiF98Ezu6hmoaWX~K6s80cF5CGFOgxnutdokoEGOvE5QbZttibk~MicLLvEYM32onm1Z37Wxm~QNh-T9Z-TtMWVIXG6mRavYcnYgVBe6wjaj7jT8a3uklh6FcZRoeUkkoxwmERKuh1JcAYVh6Zf83tkTlMN~Ag__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Neutralizing anti-VEGF mAb blocks CD40-induced MM cell migration.
MM cells were subjected to transmigration assay and exposed for 8 hours (RPMI 8226 cells) or 16 hours (SV cells) to CD40 activation by sCD40L (0 or 10 μg/mL [μM]), in the presence or absence of neutralizing anti-VEGF mAb (0-5 μg/mL [μM]) added to the lower chamber of the transwell. Migrated cells collected from the lower chamber were counted. Enhanced transmigration of cells induced by sCD40L was abrogated by neutralizing anti-VEGF mAb. (■) RPMI 8226; (▪) SV.
