Effect of protein tyrosine kinase and protein tyrosine phosphatase inhibitors on ADP-induced thromboxane A₂generation.

Washed human platelets were activated with ADP (10 μM) plus fibrinogen at 37°C with stirring in the presence or absence of inhibitor (as labeled). Reaction in the absence of the inhibitor is used as a control. The aggregation tracings are representative of the data from 3 independent experiments, and the corresponding thromboxane B₂ data were measured in experiments performed in duplicate (n = 3) and analyzed as in Figure 1.