Fig. 3.
Fig. 3. Quantitative real-time RT-PCR analysis of the binding of HCV in X plasma to human platelets and cell lines. / Human platelets (1 × 106) or Molt-4 or U937 cells (1 × 105) were incubated with 100 μL PBS or of X plasma (1.7 × 105 IU HCV). The amounts of HCV RNA and 18S rRNA (internal control) were subsequently determined in total RNA extracted from cells or from the buffer used for the final wash of cells. Data are presented as Ct values (aCt value of 40 indicates no amplification; decreasing Ct values indicate increasing concentrations of amplicon) in panel A and as absolute total amounts of HCV RNA (IU) in panel B, and are means ± SD of 5 separate experiments.

Quantitative real-time RT-PCR analysis of the binding of HCV in X plasma to human platelets and cell lines.

Human platelets (1 × 106) or Molt-4 or U937 cells (1 × 105) were incubated with 100 μL PBS or of X plasma (1.7 × 105 IU HCV). The amounts of HCV RNA and 18S rRNA (internal control) were subsequently determined in total RNA extracted from cells or from the buffer used for the final wash of cells. Data are presented as Ct values (aCt value of 40 indicates no amplification; decreasing Ct values indicate increasing concentrations of amplicon) in panel A and as absolute total amounts of HCV RNA (IU) in panel B, and are means ± SD of 5 separate experiments.

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