Fig. 2.
Fig. 2. Expression of CD81 in human cell lines and platelets. / (A) Flow cytometric analysis of CD81 expression by Molt-4 cells, U937 cells, and human platelets. Fluorescence profiles are shown as open for cells labeled with a mAb to CD81 and as shaded for cells labeled with an IgG isotype control mAb. Results are representative of 3 to 5 separate experiments. (B) RT-PCR analysis of CD81 mRNA in Molt-4 (lane 1) and U937 (lane 2) cells. Total RNA from the 2 cell types was also analyzed with primers specific for β-actin mRNA as an internal control; given the similar molecular sizes of the CD81 and β-actin amplicons, 2 separate amplifications were performed with the same cDNA sample. Lanes labeled −ve and +ve represent reaction mixtures in which total RNA was omitted or replaced with a plasmid encoding human CD81, respectively. The leftmost and rightmost lanes contain molecular size markers.

Expression of CD81 in human cell lines and platelets.

(A) Flow cytometric analysis of CD81 expression by Molt-4 cells, U937 cells, and human platelets. Fluorescence profiles are shown as open for cells labeled with a mAb to CD81 and as shaded for cells labeled with an IgG isotype control mAb. Results are representative of 3 to 5 separate experiments. (B) RT-PCR analysis of CD81 mRNA in Molt-4 (lane 1) and U937 (lane 2) cells. Total RNA from the 2 cell types was also analyzed with primers specific for β-actin mRNA as an internal control; given the similar molecular sizes of the CD81 and β-actin amplicons, 2 separate amplifications were performed with the same cDNA sample. Lanes labeled −ve and +ve represent reaction mixtures in which total RNA was omitted or replaced with a plasmid encoding human CD81, respectively. The leftmost and rightmost lanes contain molecular size markers.

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