Fig. 4.
![Fig. 4. Effect of protein synthesis inhibitors on ceramide metabolism in HL-60/VCR cells. / Cells (500 000/well) were seeded in 6-well plates in complete medium containing 1.0 μCi [3H]palmitic acid, and incubated for 6 hours with the agents indicated: emetine (0.5 μg/mL); hygromycin B (80 μg/mL); GM-CSF (1.0 nM); DT388–GM-CSF (1.0 nM), or combinations. Total cellular lipids were extracted, and [3H]ceramide was quantitated. The concentrations of emetine and hygromycin B are those that promote a 50% suppression of protein synthesis in HL-60/VCR cells at approximately 6 hours. Each experimental group represents the average of 3 cultures with variation less than 10%.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/6/10.1182_blood.v98.6.1927/7/h81811534004.jpeg?Expires=1750620824&Signature=ZB2Spu5gnBcVH4-m3fTeGVmXP8YfESXHLGg8gJf2L32Z5wwViH-hCaNFSuQp~OkrwSRoita8I5-vsFBLU3iowAFkyM4PKslfHMjchZ6UtDa6Edi0Wva1WN~EGSIm4taHHASQrXwhGKV0VUxS4YLVm0-yUM2p8b2m5z4Sa3bI7PGOFEg~awJwf6qr8gjtRYEO3x8ErRPTRb20HonquS6-dfzPfqIaHT577iM2fpnDWcvAahvWuW-HOzG1Cil22zlkXysRBguuPkAqQhnL2xuNcbSnCq7yQ1F3ggJzltbSjwHEBu5bHoRFOovjVbSs5du2g4i8yKljgP2j9en9X0-Y7g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of protein synthesis inhibitors on ceramide metabolism in HL-60/VCR cells.
Cells (500 000/well) were seeded in 6-well plates in complete medium containing 1.0 μCi [3H]palmitic acid, and incubated for 6 hours with the agents indicated: emetine (0.5 μg/mL); hygromycin B (80 μg/mL); GM-CSF (1.0 nM); DT388–GM-CSF (1.0 nM), or combinations. Total cellular lipids were extracted, and [3H]ceramide was quantitated. The concentrations of emetine and hygromycin B are those that promote a 50% suppression of protein synthesis in HL-60/VCR cells at approximately 6 hours. Each experimental group represents the average of 3 cultures with variation less than 10%.
