Fig. 5. IL-2Rα, but not IL-2Rβ or... | American Society of Hematology

Fig. 5.

$Fig. 5. IL-2Rα, but not IL-2Rβ or IL-2Rγ, is enriched in lipid rafts. / CTLL-2 cells were left untreated or were stimulated for 10 minutes with IL-2 before lysis. Lysates were subjected to discontinuous sucrose density gradient ultracentrifugation, and fractions (Fr) were collected and separated by SDS-PAGE. Localization of IL-2α, IL-2Rβ, and IL-2Rγ chains was analyzed by immunoblotting. The final lane in each blot consists of immunoprecipitations of IL-2Rα, IL-2Rβ, or IL-2Rγ chains as positive controls for immunoblotting.$

IL-2Rα, but not IL-2Rβ or IL-2Rγ, is enriched in lipid rafts.

CTLL-2 cells were left untreated or were stimulated for 10 minutes with IL-2 before lysis. Lysates were subjected to discontinuous sucrose density gradient ultracentrifugation, and fractions (Fr) were collected and separated by SDS-PAGE. Localization of IL-2α, IL-2Rβ, and IL-2Rγ chains was analyzed by immunoblotting. The final lane in each blot consists of immunoprecipitations of IL-2Rα, IL-2Rβ, or IL-2Rγ chains as positive controls for immunoblotting.

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