Induction of E-selectin expression on lung microvascular endothelial cells.

Lung microvascular endothelial cells (LEISVO) and bone marrow–derived endothelial cells (STR-12) were exposed to nicotine for 4 hours. Expression of CD44, VCAM, and E-selectin was evaluated by FACS analysis. Adhesion molecule expression on untreated cells (dark field) was compared with that on nicotine-treated cells (transparent field).