Fig. 3.
Fig. 3. Fluorescence microscopy of FITC-FN assembly in response to different agonists on FN- or VN-coated coverslips. / After a 15-minute adherence phase, coverslips were incubated for 1 hour with 20 mg/L FITC-FN in the presence of no agonist (0); 0.5, 10, or 20 μM LPA; 10 μM ADP; 20 μM S1P; or 1 U/mL thrombin. Phase contrast microscopy (not shown) revealed monolayers of platelets under all conditions. Bar = 10 μm.

Fluorescence microscopy of FITC-FN assembly in response to different agonists on FN- or VN-coated coverslips.

After a 15-minute adherence phase, coverslips were incubated for 1 hour with 20 mg/L FITC-FN in the presence of no agonist (0); 0.5, 10, or 20 μM LPA; 10 μM ADP; 20 μM S1P; or 1 U/mL thrombin. Phase contrast microscopy (not shown) revealed monolayers of platelets under all conditions. Bar = 10 μm.

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