Fig. 2.
Fig. 2. Fluorescence microscopy of assembling FITC-FN on LPA-treated washed platelets, after a 15-minute adherence phase. / Platelets on FN-coated (upper panel) or VN-coated (lower panel) coverslips were stimulated with 1 μM LPA in the presence of FITC-FN, 20 mg/L, and fixed after an additional 10, 20, 30, and 60 minutes. Note the increase in number of fibrils and intensity of FITC-FN fluorescence with increased times. Phase contrast microscopy (not shown) revealed monolayers of platelets on both substrates at all time points. Bar = 10 μm.

Fluorescence microscopy of assembling FITC-FN on LPA-treated washed platelets, after a 15-minute adherence phase.

Platelets on FN-coated (upper panel) or VN-coated (lower panel) coverslips were stimulated with 1 μM LPA in the presence of FITC-FN, 20 mg/L, and fixed after an additional 10, 20, 30, and 60 minutes. Note the increase in number of fibrils and intensity of FITC-FN fluorescence with increased times. Phase contrast microscopy (not shown) revealed monolayers of platelets on both substrates at all time points. Bar = 10 μm.

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