Fig. 5.
Fig. 5. pLL20/DNAct complexes, but not pLL211/DNA, fix mouse complement C3 independently of immunoglobulins. / Biotin-pLL20/DNAct or biotin-pLL211/DNAct complexes were immobilized on streptavidin magnetic beads and incubated in mouse serum at 37°C for 5 minutes. Beads were isolated, washed in isotonic buffer, and boiled in Laemelli buffer to isolate the bound proteins. Dot blots were prepared on nitrocellulose membrane, and proteins were identified by antibody blotting and radiography. (A) C3 binding to pLL/DNAct complexes in normal mouse serum. (B) C3 binding to pLL20/DNAct complexes in normal or heat-inactivated serum. (C) Total mouse immunoglobulins binding to pLL/DNAct complexes in normal serum. Bead control represents serum-treated beads. Similar results were obtained using pLL/DNAcp complexes (data not shown).

pLL20/DNAct complexes, but not pLL211/DNA, fix mouse complement C3 independently of immunoglobulins.

Biotin-pLL20/DNAct or biotin-pLL211/DNAct complexes were immobilized on streptavidin magnetic beads and incubated in mouse serum at 37°C for 5 minutes. Beads were isolated, washed in isotonic buffer, and boiled in Laemelli buffer to isolate the bound proteins. Dot blots were prepared on nitrocellulose membrane, and proteins were identified by antibody blotting and radiography. (A) C3 binding to pLL/DNAct complexes in normal mouse serum. (B) C3 binding to pLL20/DNAct complexes in normal or heat-inactivated serum. (C) Total mouse immunoglobulins binding to pLL/DNAct complexes in normal serum. Bead control represents serum-treated beads. Similar results were obtained using pLL/DNAcp complexes (data not shown).

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