Two-step translocation of PLCγ2.

Under basal conditions (A), PLCγ2 is cytosolic and not phosphorylated, the transmembrane adapter protein LAT is not phosphorylated, and the level of PI 3,4,5-P3 is minimal. After activation of GPVI (B), LAT becomes phosphorylated on tyrosine and associates with the C-terminal domain of PLCγ2. In addition, GPVI stimulates PI 3-kinase, leading to the formation of PI 3,4,5-P3 in the membrane. The localization of PLCγ2 in the membrane is stabilized by binding of PI 3,4,5-P3 to the PH domain of PLCγ2 (C).