Analysis of the effect of LZ-RANKL or sRANKL on the differentiation of osteoclast precursor cells.

(A) The dose-dependency of LZ-RANKL was examined by measuring TRAP activity in liquid culture. (B) c-Fms⁺RANK⁻cells were cultured in methylcellulose in the presence of 1000 ng/mL LZ-RANKL or 25 ng/mL sRANKL with 100 ng/mL M-CSF for 6 days. Then single colonies were collected, and the frequency of TRAP⁺cells was examined.