Fig. 6.
Fig. 6. Intact DCs form significant amounts of 5-HETE and LTB4. / HPCs were maintained as described in Figure 1 in medium supplemented in standard medium (open column) or standard medium plus TGF-β–1 (closed column). The cell populations were assayed for production of (A) 5-HETE and (B) LTB4 after the addition of 10 μmol/L Ca++ ionophore A 23187 and 40 μmol/L arachidonic acid as described.15 Data represent the mean of triplicate dishes prepared in parallel with a maximum SD of 4.7% for 5-HETE and 20% for LTB4. Similar data were obtained in a second experiment.

Intact DCs form significant amounts of 5-HETE and LTB4.

HPCs were maintained as described in Figure 1 in medium supplemented in standard medium (open column) or standard medium plus TGF-β–1 (closed column). The cell populations were assayed for production of (A) 5-HETE and (B) LTB4 after the addition of 10 μmol/L Ca++ ionophore A 23187 and 40 μmol/L arachidonic acid as described.15 Data represent the mean of triplicate dishes prepared in parallel with a maximum SD of 4.7% for 5-HETE and 20% for LTB4. Similar data were obtained in a second experiment.

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