Fig. 5.
Fig. 5. FACS dot-blot analyses of 5-LO protein-expressing cell populations derived from HPCs. / The y axes of the dot-blots show that TGF-β–1 increases the frequency of cells with high levels of CD80, CD86, CD83, CD1a, and Lag, and strongly reduces the frequency of cells expressing mannose receptor and CD14. HPCs were maintained in standard medium (S, left columns) or standard medium plus TGF-β–1 (S + T, right columns) for 15 days (Figure 1). 5-LO staining was performed as described in “Materials and methods.” Panels A and B represent 2 independent experiments. Secondary antibody for 5-LO staining was goat antirabbit IgG-FITC, except for HLA-DR–expressing and CD14-expressing cells in panel B, in which the secondary antibody was donkey antirabbit IgG-PE.

FACS dot-blot analyses of 5-LO protein-expressing cell populations derived from HPCs.

The y axes of the dot-blots show that TGF-β–1 increases the frequency of cells with high levels of CD80, CD86, CD83, CD1a, and Lag, and strongly reduces the frequency of cells expressing mannose receptor and CD14. HPCs were maintained in standard medium (S, left columns) or standard medium plus TGF-β–1 (S + T, right columns) for 15 days (Figure 1). 5-LO staining was performed as described in “Materials and methods.” Panels A and B represent 2 independent experiments. Secondary antibody for 5-LO staining was goat antirabbit IgG-FITC, except for HLA-DR–expressing and CD14-expressing cells in panel B, in which the secondary antibody was donkey antirabbit IgG-PE.

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