Fig. 1.
Fig. 1. Appearance of 5-LO and FLAP transcripts during DC differentiation of HPCs. / Highly purified HPCs (more than 98%) were prepared from CB as described in “Materials and methods.” The cells were maintained in culture in the presence of SCF/GM-CSF/TNF-α (open columns), referred to as standard medium, or in the presence of SCF/GM-CSF/TNF-α/TGF-β–1 (closed columns), referred to as standard medium plus TGF-β–1, for increasing time periods (days, d). Transcript levels of (A) 5-LO and (B) FLAP were determined by RT-PCR analyses as described.4 Data are expressed as ratios of densitometry units of 5-LO (27 cycles) or FLAP (25 cycles) transcripts relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcripts (22 cycles). nd indicates not detectable.

Appearance of 5-LO and FLAP transcripts during DC differentiation of HPCs.

Highly purified HPCs (more than 98%) were prepared from CB as described in “Materials and methods.” The cells were maintained in culture in the presence of SCF/GM-CSF/TNF-α (open columns), referred to as standard medium, or in the presence of SCF/GM-CSF/TNF-α/TGF-β–1 (closed columns), referred to as standard medium plus TGF-β–1, for increasing time periods (days, d). Transcript levels of (A) 5-LO and (B) FLAP were determined by RT-PCR analyses as described.4 Data are expressed as ratios of densitometry units of 5-LO (27 cycles) or FLAP (25 cycles) transcripts relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcripts (22 cycles). nd indicates not detectable.

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