Metabolic inhibitors induce in a time-dependent manner the shedding of L-selectin.
(A) Kinetics of the effect of metabolic inhibitors on the expression of L-selectin (●) and CD11b (■) in neutrophils. Cells were cultured in the presence or absence of metabolic inhibitors at concentrations described in Figure 4 for different periods. The rMFI of L-selectin and CD11b was related to the expression by cultured cells in medium in each time. A representative experiment of 3 is shown. (B) Quantification of neutrophil-shed L-selectin induced by NSAIDs. Neutrophils were incubated with azide+2-DG, PMA, and medium alone for 30 minutes at 37°C. After centrifugation, neutrophil-free supernatants were tested for sL-selectin by ELISA as described in “Materials and methods.” Values represent the mean (ng/106 cells) ± SD from 3 independent experiments. * = P < .01 versus medium, by Student pairedt test.
