Cell surface tissue factor pathway activity in response to 7α- or 7β-hydroxycholesterol, 7-ketocholesterol, 7β-hydroperoxycholesterol, or t-butyl hydroperoxide.

(A) Oxidized LDL, native LDL (200 μg cholesterol/mL), 7α- or 7β-hydroxycholesterol (10 μmol/L) or 7-ketocholesterol (10 μmol/L) was added to rat aortic smooth muscle cells, and surface tissue factor pathway activity was measured 4 hours later. 7β-Hydroperoxycholesterol (10 μmol/L, B) or t-butyl hydroperoxide (25 μg/mL, C) was added to rat aortic smooth muscle cells, and surface tissue factor pathway activity was measured 2 hours later. Data represent means ± SD of 4 wells per treatment. Data are from experiments representative of multiple experiments at 2 and 4 hours.