Effect of oxidized LDL on tissue factor mRNA and protein.

(A) Tissue factor (TF) mRNA from rat aortic smooth muscle cells was assessed by Northern blot analysis 90 minutes after the addition of fetal bovine serum (FBS, 10%), or oxidized LDL at 0, 50, 100, and 200 μg cholesterol/mL. (B) Tissue factor surface protein immunoprecipitated from 50 μg of cell lysate of human aortic smooth muscle cells was assessed by Western blot analysis 4 hours after “no treatment” (NT; control), FBS (10%), or oxidized LDL (100 or 200 μg cholesterol/mL). Glyceraldehye 3-phosphate dehydrogenase (GAPDH) was assessed as an internal control to confirm equal loading in each lane for Northern blot analysis.