Fig. 6.
![Fig. 6. 1α,25 Dihydroxyvitamin D3-mediated repression of IL-6 promoter activity in KS cells. / Shown is a representative phosphorimager analysis of pIC225 activity cotransfected with VDR expression vector. The IL-6 promoter construct, containing 225 bp of IL-6 gene 5′-flanking DNA sequence fused to the CAT reporter gene, was cotransfected with VDR expression vector. The CAT activity of this construct in the presence of increasing amounts of 1α,25 dihydroxyvitamin D3 is shown. The IL-6 promoter is minimally inactive in KS Y-1 cells (left-most lane) in the absence of TPA. Numbers above the image were obtained by phosphorimager quantitation of CAT activity in which acetylated products (upper spots) are expressed as a percentage of the total [14C].](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/9/10.1182_blood.v96.9.3188/5/h82100324006.jpeg?Expires=1750793689&Signature=uLSmPhXs-zpvECU0ICxlZ2vAnHDwpUKlnUSum8aJaibNPfKcC2eiyn9ngcfTKgjQ6XzmQ0lyLWK~6rQwsLU-P0tVls8EJvkAE2RhALoi1DbKjN2yxICalbNmRAuvRJqm-RAzMePDGh2JO72tpysKa3FBSo2mzmUIbIJgnfSUspca6uc-QChpzDcAyQ5Rl487C8JntViibPT2Jj8xrLNsZwc7VTSohgkjuerk-n0ElrO9Ym2GhrkRH~Jjc5DeUpUMj4wjiIv8Wl6QM3DlKCctiYrxTj36Zo0eB4tAsdjR2exKSv6Ch2AB~S2yqIyEDoEtQkdMkK~z2KQ4GMLHhJTrRA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
1α,25 Dihydroxyvitamin D3-mediated repression of IL-6 promoter activity in KS cells.
Shown is a representative phosphorimager analysis of pIC225 activity cotransfected with VDR expression vector. The IL-6 promoter construct, containing 225 bp of IL-6 gene 5′-flanking DNA sequence fused to the CAT reporter gene, was cotransfected with VDR expression vector. The CAT activity of this construct in the presence of increasing amounts of 1α,25 dihydroxyvitamin D3 is shown. The IL-6 promoter is minimally inactive in KS Y-1 cells (left-most lane) in the absence of TPA. Numbers above the image were obtained by phosphorimager quantitation of CAT activity in which acetylated products (upper spots) are expressed as a percentage of the total [14C].
