Role of perforin and FasL in CD4⁺T-cell–mediated GVL.

We injected B6 mice with MMB3.19 cells and lethally irradiated them the next day. (A) The recipient mice received ATBM cells alone (n = 8) or ATBM cells in combination with 2 × 10⁶MMB3.19-primed CD4⁺ T cells from either wt(n = 10), pfp^o (n = 10), orgld (n = 9) donor B6 mice. (B) The mice received ATBM cells alone (n = 7) or ATBM cells in combination with 1 × 107MMB3.19-primed CD4-enriched wt splenocytes (n = 7) or 5 × 10⁷gld CD4-enriched splenocytes (n = 6). The survival of mice was monitored daily until termination of the experiment. The results in panels A and B represent pooled data from 2 similar experiments and a single experiment, respectively.