Effects of Ub on IL-6–induced expression of apoptosis-regulating genes.

(A) KT-3 cells were rhIL-6–deprived for 12 hours and then cultured with 10 ng/mL rhIL-6. To examine the effects of Ub, the cells were pretreated with 100 μg/mL Ub for the last 3 hours of the starvation period and were cultured with Ub during the test period. Total cellular RNA was extracted at the times indicated and subjected to Northern blot analysis. (B) Expression of Bcl-2 protein during the culture with or without Ub treatment. Total cellular lysates were isolated from the cultured cells at the times indicated, and the expression levels of Bcl-2 proteins were examined by Western blot analysis.