Fig. 2.
HMEC-1 cells adhere to iodoacetamide-inactivated factor XIIIa, but not factor XIIIb.
Cell adhesion assays were conducted using immobilized factor XIIIb (black bars), iodoacetamide-inactivated factor XIIIa (gray bars), or thrombin-activated factor XIIIa (white bars). HMEC-1 cells were allowed to attach for 40 minutes at 37°C in 5% CO2 to wells coated with the indicated concentrations of the different factor XIII preparations. Adherent HMEC-1 cells were fixed, stained with toluidine blue, solubilized with 2% SDS, and assayed for absorbance at 650 nm. Background binding to uncoated wells was subtracted from each value. Each bar represents the mean of 6 determinations ± SD.
