Immunoblot analyses of tissue and cell lysates.

SDS lysates from tissues (A, B, C) or epithelial and hematopoietic cell lines (D, E, F) were resolved by 10% SDS-PAGE and immunoblotted with the 105A5 (A, D), 103B2/9E10 (E), N6B6 (B, F), or 67D2 (C) Mabs. Tissues analyzed on blots (A, B, C) were heart (lane 1), pancreas (lane 2), colon (lane 3), kidney (lane 4), and spleen (lane 5). Cell lines (D, E, F) were RPMI-1788 (lane 1), HeLa (lane 2), U937 (lane 3), 293T (lane 4), KG1A (lane 5), and Calu-1 (lane 6).