Expression of human survivin mRNA forms.
Prior to separation by agarose gel electrophoresis, nested RT-PCR was performed on mRNA derived from the following human cell lines and tissues: 1. K562 leukemia cell line; 2. Jurkat T-cell line; 3. germ-cell tumor derived cell line; 4. teratocarcinoma cell line; 5. neuroblastoma cell line; 6-9. malignant tumor from patients with MALT lymphoma; 10. AS19 melanoma cell line; 11. Del leukemia cell line; 12. SW620 colon cancer cell line; 13. Hep3B hepatoma cell line; 14. Ht1080 neurofibrosarcoma cell line; 15. A431 carcinoma cell line; 16. Raji leukemia cell line. (A) Ethidium bromide-stained agarose gel of nested RT-PCR products generated with oligonucleotide primers hsrv2861 and hsrv5431. Transcripts correspond to full-length murine survivin140. (B) Southern blot using32P-labeled hsrv2888 to detect specific nested RT-PCR products generated using oligonucleotide primers hsrv2861 and hsrv5159. Several cell lines express the specific transcript corresponding to murine survivin121, whereas only the neuroblastoma cell line expresses a transcript corresponding to murine survivin40.
