Murine survivin gene and cDNAs.

Exons 1 to 4 are denoted in red, light green, blue, and green, respectively. Intron 3 is gray, and the 3′ flanking region is pink. (A) Restriction map of murine survivin gene. The arrow indicates the ATG translational start site. Restriction enzyme sites are shown: K (Kpn1); Stu (Stu1); Spe (Spe1); B (BglII); RV (EcoRV); A (Asc1); S (Sac1); P (Pml1); Apa (Apa1); Bc (Bcl1). Location of DNA probes used for Southern blots are shown. (B) Exon organization of the 3 distinct cDNAs encoding the predicted survivin proteins. The arrow indicates the start of translation. The heavy black vertical line represents the end of the coding region. Survivin₁₄₀ is derived from all 4 exons. Survivin₄₀ is generated by removal of exon 2-derived sequences, with a resultant in-frame stop codon, and a truncated protein. Survivin₁₂₁ is derived from exons 1 to 3, and a short part of intron 3, the latter being presumably retained during pre-mRNA processing.