Transient in vivo inhibition of the murine erythrocyte Cl− conductance.
The mice were intravenously dosed with NS1652 (50 mg/kg) or vehicle (5% W/v cremophore) at time 0. At 1, 5, 30, and 120 minutes after injections, 3 mice were killed and their blood collected in heparin and immediately centrifuged. The vehicle-injected animals were killed after 1 minute. The packed erythrocytes were separated from the plasma and stored on ice until use (1-2.5 hours). Erythrocytes from uninjected control animals were processed similarly. Immediately before analysis, the packed cells were resuspended in 1 vol experimental salt solution and centrifuged, and 100 μL were transferred to 3 mL experimental solution for recording of hyperpolarization induced by a fixed valinomycin concentration (5 ∗ 10−7 mol/L). The individual blood samples were analyzed at random. ○ indicates control animals; □ indicates cremophore-injected animals; ⧫ indicates NS1652-injected animals. n = 3 for each group. Data are means ± SD. Broken line indicates the mean of control and cremophore-injected animals.
