Erythropoietin 5′-UTR recruits a methyl-CpG–binding protein.

Electrophoretic mobility shift assays were performed with methylated and unmethylated CG11 and EPO236 as probes and competitors. [³²P]-end-labeled probes were incubated with nuclear extracts and, where indicated, with excess unlabeled competitors. (A) CG11 probe. (B) EPO236 probe. U, unmethylated; M, methylated probe or competitor. Probe identity and methylation status are noted below the panels, and competitors and sources of nuclear extracts are noted above the panels. (arrow) Methyl-CpG–binding complex. (asterisks) Complexes formed on unmethylated probes blocked by methylation. (arrowheads) Protein complexes formed on unmethylated and methylated probes, whose formation is blocked by methylated and unmethylated competitors.