Cytokine regulation of IPP-induced chemokine expression as determined by ELISA.

Vδ2⁺–enriched cultures were established by stimulating with IPP and culturing in IL-2 for 4 weeks. Cells were then activated again with IPP (IPP × 2) in the presence of IL-2 alone and IL-2 +IL-4, +IL-10, +TGFβ, and +INFγ. Culture supernatants were harvested 48 hours later, and MIP-1α and MIP-1β were released into the medium as determined by ELISA. Data shown represent the mean ± SD of 3 healthy donors.