pFALP and sFALP both enable responses of PMN to LPS.

PMN adhesion was measured as described in “Methods” using plasma- or serum-derived FALP preparations incubated with LPS (10 ng/mL). The LBP concentrations of pFALP and sFALP were 14.4 and 16.9 μg/mL before dilution, respectively. 3 different dilutions (1/50, 1/100, and 1/200 that correspond to ∼0.3, 0.15, and 0.07 μg/mL of LBP, respectively) of FALPs were tested. Each data point and error bar represents the average and the standard deviation from triplicate sample preparations. Controls included pFALP or sFALP incubated with PMN without LPS (pFALP-LPS or sFALP-LPS), and PMN incubated without pFALP or sFALP (buf+LPS) or with buffer alone (buf-LPS). The experiments were repeated twice with similar results.