Fibrinogen coprecipitates with components of FALP in sandwich ELISAs.

Monoclonal antibodies against each component of FALP were coated on the plastic surface of Terasaki plates as shown on the X-axis, and sandwich ELISAs were performed as described in Methods. After NHP (diluted 1/200 in PBS) was incubated and washed, polyclonal antifibrinogen antibody was added to detect fibrinogen co-associated with the test antigen. Antitissue factor pathway inhibitor (TFPI) was used as a control antibody. The increase in arbitrary units of fluorescence indicated on the Y-axis is proportional to the amount of bound antifibrinogen antibody. Control experiments were run to demonstrate that each of the monoclonal antibodies bound its own antigen, which could be detected with the corresponding polyclonal antibodies (data not shown). The experiment was repeated twice with similar results. Error bars represent the standard deviations of the triplicate measurements.