Fig. 3.
Fig. 3. Plasma cell differentiation in vitro and in vivo. May-Grünwald-Giemsa staining of cell cytospins at day 0 (A and C) or 6 (B and D) from fresh (A and B) or thawed (C and D) PBMNCs or of bone marrow smears (E and F) from patient RP5. From day 0, fresh or thawed PBMNCs were cultured for 6 days in 10% FCS RPMI 1640. After thawing, plasma cells (CD138+) and B cells (CD20+) were removed, as described in Materials and Methods. The CD19+ plasmablastic population (C) accounted for 95% of cells; the remaining cells were T lymphocytes and monocytes. (Original magnification × 1,000.)

Plasma cell differentiation in vitro and in vivo. May-Grünwald-Giemsa staining of cell cytospins at day 0 (A and C) or 6 (B and D) from fresh (A and B) or thawed (C and D) PBMNCs or of bone marrow smears (E and F) from patient RP5. From day 0, fresh or thawed PBMNCs were cultured for 6 days in 10% FCS RPMI 1640. After thawing, plasma cells (CD138+) and B cells (CD20+) were removed, as described in Materials and Methods. The CD19+ plasmablastic population (C) accounted for 95% of cells; the remaining cells were T lymphocytes and monocytes. (Original magnification × 1,000.)

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