Fig. 2.
Cleavage of gelsolin in apoptotic neutrophils. Whole cell extracts obtained from 5 × 105 neutrophils treated with (lanes 2 through 5) or without (lane 1) TNF- plus cycloheximide (TNF/CHX) for the time indicated were subjected to SDS-PAGE (10% gel) and immunoblotting using a monoclonal anti-gelsolin antibody.