Agarose gel electrophoresis of Molt-4 DNA. Molt-4 cells were untreated or treated with 8 nmol/L GW1843 for 48 hours and purified live, blebbed, and dead cell populations isolated on histopaque gradients as described in Materials and Methods. Cellular DNA was prepared and resolved by electrophoresis in 1% agarose gels gel as described in Materials and Methods. Lane 1, one kb ladder; lane 2, live fraction from untreated control cells after resolution by histopaque gradients; lane 3, GW1843-treated, normal-appearing cells after histopaque gradients (fraction contains approximately 6% dead cells); lane 4, GW1843-treated, blebbed cells after gradients; lane 5, GW1843-treated, dead cells after gradients; lane 6, untreated cells before gradients (fraction contains 93% normal cells and 7% dead cells); lane 7, GW1843-treated cells before gradients (26% normal cells, 52% blebbed, and 22% dead). The gel is from a representative experiment from three separate determinations.